Fluorescence Life Time Imaging, PicoQuant + Confocal Microscope

The FILM system, DCS 120, Becker & Hickl  includes two pulsed picosecond lasers (405nm , 470nm), two confocal single photon avalanche photodiode (SPAD) detectors, adapted to an Olympus IX-81 fully motorized inverted research microscope.

  • FLIM imaging (two excitation channels, two emission channels)
  • FCS (including image cross-correlation)
  • Regular 2-channel confocal imaging